Mcherry-muc2
WebIn the colon, where there are as many as 10 12 bacteria per gram of stool, Muc2 forms an outer non-attached mucus layer inhabited by microbes and a sterile inner mucus layer that is adherent to the intestinal epithelium 90, 91. Web19 apr. 2024 · 通过使用mCherry-MUC2转基因小鼠来分选和分离GC，这些作者生成了转录组和蛋白质组图谱来表征小肠和结肠中的GC表达景观。单细胞转录组分析揭示了每个组织区域中几个不同的GC簇，它们可分离成两个独立的轨迹。
Mcherry-muc2
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Web25 jan. 2024 · In the mouse, Muc2 is the predominant intestinal secreted mucin in health, and deficiency of Muc2 leads to spontaneous inflammation and susceptibility to infection. … Web16 apr. 2024 · ScRNA-seq trajectory analysis of mCherry-Muc2 cells showed that goblet cells segregate into two separate trajectories: one is enriched with canonical markers …
Web11 jan. 2024 · The mCherry-Muc2 mouse line was kindly shared by Gunnar C. Hansson, University of Gothenburg, Sweden. X-Gal Staining. Fresh entire colons (from anus to … WebMS2 Mutation MS2: V75E/A81G Promoter human UbC Tags / Fusion Proteins V5 (N terminal on insert) mCherry (C terminal on insert) Cloning Information Cloning method …
Web28 mei 2024 · Please note that the interaction of S.Tm with this mCherry-MUC2 mucus does not reflect the natural interaction with the inner mucus of the distal colon. Previous … Web16 aug. 2024 · Europe PMC is an archive of life sciences journal literature.
Weban mCherry–human MUC2–expressing transgenic mouse (RedMUC298trTg)revealedsecretedMuc2 onlyintreatedtissues(Fig.1D).Asmucusexpands …
Web21 mrt. 2024 · MUC2 (Mucin 2, Oligomeric Mucus/Gel-Forming) is a Protein Coding gene. Diseases associated with MUC2 include Lung Oat Cell Carcinoma and Small Cell … cod heis testWeb21 okt. 2024 · 17 The mucin Muc2 is a major constituent of the mucus layer that covers the intestinal epithelium and creates 18 a barrier between epithelial cells and luminal … cod hellhoundWeb10 mrt. 2024 · TLR-ligands induce Muc2 secretion in specific GCs Intestinal GCs are more functionally heterogeneous than previously expected (reflected processes happening ... RedMUC298trTg cells: magnified x/z-axis cross-section areas indicated by white boxes (lower), LPS/P3CSK4 (green), mCherry-MUC2 (reddish). (B) WT with or without Casp. caltech advancement and alumni relationsWebmCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004). caltech address 1200Web10 aug. 2024 · mCherry2 is a basic (constitutively fluorescent) red fluorescent protein published in 2024, derived from Discosoma sp.. It has very low acid sensitivity. Oligomerization Organism Molecular Weight … caltech address and phone numberWeb1 sep. 2024 · These functions depend on the intestinal epithelium that constitutes the second-largest epithelial surface area of more than 30m2in the human body (Helander and Fandriks, 2014). The intestinal epithelial surface is expanded by millions of special structures, crypt-villus units in the small intestine or crypt units in the large intestine (Fig. … caltech 22 30 roundsWebUsing RedMUC2-Tg mice, which express a human MUC2 fused to mCherry, and originally developed by Hansson and colleagues, Citation 51 Nystrom et al. showed an intercrypt … cod hemograma