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Dsred-express2

WebOriGene Anti-DsRed-Express2 Monoclonal (OTI1C4), TrueMAB™, Catalog # TA180014. Tested in Western Blot (WB) applications. Supplied as 100 µL purified antibody (3.64 … Web产品应用. FDA 是一种非荧光性疏水性荧光素衍生物，它可以穿透细胞膜进入细胞，通过细胞内酯酶催化水解二乙酸酯基团产生具有高强度的荧光产物荧光素。. 荧光分子在具有完整细胞膜的细胞内积累，因此这种绿色荧光可以作为一种细胞活力的标签。. 不具有 ...

Frontiers A New Suite of Plasmid Vectors for Fluorescence-Based ...

WebDsRed-Express2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2008, derived from Discosoma sp.. It is reported to be a rapidly-maturing protein. We would like to show you a description here but the site won’t allow us. Allison Berman - DsRed-Express2 :: Fluorescent Protein Database Wen Tao - DsRed-Express2 :: Fluorescent Protein Database Hal E Broxmeyer - DsRed-Express2 :: Fluorescent Protein Database References and fluorescent proteins attributed to Dibyendu Bhattacharyya at … Browse. If you just want to browse the database without searching something … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … References and fluorescent proteins attributed to Daniel E Strongin at … A Rapidly Maturing Far-Red Derivative of DsRed-Express2 for Whole-Cell … WebWild-type DsRed matures very slowly. We overcame this problem by using directed evolution to create the first rapidly maturing DsRed variants, one of which is marketed commercially as DsRed-Express. More recent work yielded a noncytotoxic variant called DsRed-Express2, as well as a far-red variant called E2-Crimson. john chapter 1 notes https://soluciontotal.net

DsRed-Express :: Fluorescent Protein Database

WebFeb 12, 2011 · DsRed-Express2 was reported to be the best variant derivative of DsRed. The fluorescence maturation, expression, photostability and photoxicity were much more improved compared with DsRed . When cells were transfected with plasmids encoding DsRed-Express2 alone, there are also 23.5% of red fluorescent cells became shriveled … WebDec 5, 2005 · Directed evolution of DsRed-Express2 yielded the color variants E2-Orange, E2-Red/Green, and E2-Crimson. These variants can be used to label whole cells for … WebPlasmid pDL278_P23-DsRed-Express2 from Dr. Robert Shields's lab contains the insert DsRed-Express2 and is published in Appl Environ Microbiol. 2024 May 17. pii: AEM.00620-19. doi: 10.1128/AEM.00620 … john chapter 1 nlt

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Category:Addgene: pSIR-DsRed-Express2

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Dsred-express2

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WebApr 3, 2009 · Background: Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. WebDsRed-Express2 Antibody (TA180014) in WB. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed-Express2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed-Express2 (TA180014, 1:2000).

Dsred-express2

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WebThe gene encoding DsRed-Express2 is fused to a constitutive promoter of GPD, providing an internal control for normalizing cell numbers in the assay. The dual fluorescent protein assay system is performed by sequentially measuring the yEGFP and DsRed-Express2 fluorescent intensity of the same sample, with the results expressed as the ratio of ... WebListed below are anti-DsRed antibodies from multiple suppliers. Native DsRed is a 28-kDa fluorescent protein originating from the Discosoma disc anemone. Recombinant DsRed is used in fluorescent tagging of proteins for detection. It emits fluorescence at an emission maximum of 583 nm. See the supplier page to learn important antibody details ...

http://pharmbiology.com/page9?product_id=642 WebNov 14, 2008 · The vector can also be used to express DsRed-Express2 in bacteria. Cells expressing DsRed-Express2 (excitation and emission maxima: 554 nm and 591 nm, …

WebTo overcome the slow maturation of DsRed, we used random and directed mutagenesis to create variants that mature 10–15 times faster than the wild-type protein.An asparagine-to-glutamine substitution at position 42 … WebOct 17, 2014 · To generate mice expressing DsRed Express2 under the neuron specific enolase promoter (NSE-DsRedE2) a cDNA construct was made by replacing the cytomegalovirus (CMV) promoter in the pCMV DsRed ...

WebFeb 1, 2024 · DsRed-Express2 and E2-Crimson coding sequences were amplified directly from plasmid DNA. Using these methods, a series of plasmids expressing various fluorescent proteins driven by P c or P psbA promoters was constructed. In some plasmids, fluorescent protein coding sequences were appended to coding sequences for TorT or …

WebDsRed-Express2 is not as bright as wild-type DsRed, so we mutagenized it to create DsRed-Max, which was B30% brighter than DsRed-Express2 and retained high solubility (Fig. 1b and Supplementary Results ). DsRed-Max had lower green emission than DsRed-Express2 (Supplementary Fig. 6 online), but was slower to mature and more photolabile … john chapter 1 nrsvWebFor full activity, add fresh S-adenosylmethionine (SAM). BssHII is typically used at 50°C, but is 75% active at 37°C. ApoI is typically used at 50°C, but is 50% active at 37°C. BsiWI is typically used at 55°C, but is 50% active at 37°C. ApaI … john chapter 1 new king james versionWebPlasmid pSIR-DsRed-Express2 from Dr. Hodaka Fujii's lab is published in PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to ... john chapter 1 ldsWebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. john chapter 1 nasbWebpIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. red fluorescent protein contains the internal ribosome entry … john chapter 1 the messageWebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA … john chapter 1 explainedjohn chapter 2 amplified bible