Biolegend cell staining buffer 420201
Web售价: ¥ 824.20 原价: ¥ 1268.00: 货号: 420241 : 品牌: Biolegend : 原产地: 分装/原装: 生产商 Web1. Obtain desired tissue (e.g. spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension in Cell Staining Buffer (BioLegend Cat. No. 420241). Note. If using in vitro stimulated cells, simply resuspend previously activated cultures in Cell Staining Buffer and proceed to Step 2. If using in vitro stimulated cells, simply ...
Biolegend cell staining buffer 420201
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WebFeb 22, 2024 · Then, cells were washed twice with cold BioLegend cell staining buffer (420241) before resuspension in Annexin V Binding Buffer (422201) at a concentration of 10 6 cells/mL. FITC Annexin V and 7-aminoactinomycin D (AAD; 420401) were added successively. DNA content was analyzed using 7-AAD. Flow cytometry was performed …
WebJan 21, 2024 · Wash cells twice with cold BioLegend cell staining buffer (cat # 420241) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10 6 cells/ml. 2. Transfer 100 ul of cell suspension in 5 ml test tube. 3. Add 5 ul of fluorochrome conjugated Annexin V. 4. Add 10 ul of PI solution (cat # 421301) or 7-AAD … WebCell Staining Buffer - Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell …
WebJul 13, 2024 · For detection of cell surface PD-L1, cells were suspended in 100 μL of cell staining buffer (#420241, BioLegend) and incubated with APC-conjugated anti-human PD-L1 antibody (#329708, BioLegend) at room temperature for 30 minutes. After washing in the staining buffer, stained cells were analyzed by FACS (BD Biosciences). ... WebImmunohistochemistry (IHC) Staining of Tissue Microarray. ... USA) at room temperature for 30 mins. After dark incubation, cells were washed with cell staining buffer (420,201, Biolegend, San Diego, USA), and then incubated with Fc receptor blocking solution (422,302, Biolegend, San Diego, USA) in dark, room temperature for 10 mins.
WebBriefly, the cells were washed with cell staining buffer (420241, BioLegend, San Diego, CA, USA) and resuspended in Annexin binding buffer. The cells were then labeled with Annexin V-FITC and PI at room temperature for 15 min in the dark. The fluorescence of stained cells was measured using a BD LSR flow cytometer and CellQuest Pro software …
WebApr 13, 2024 · RBCs were lysed using ACK Lysis Buffer (Lonza 10-548E) and the blood was further diluted at 1:100 ratio in the cell staining buffer (Biolegend, 420241). black 2 shiny oddsWebCell Staining Buffer - Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. daughtry what i want lil nas xWebFor storage longer than 4 hours, wash 1X with Biolegend Cell Staining Buffer (cat# 420241) and resuspend in 0.5 mL/tube Cell Staining Buffer, cover the tubes. Caution: … black 2 the futureWebStop cell lysis by adding 10ml Cell Staining Buffer to the tube. Centrifuge for 5 minutes at 350xg and discard supernatant. Repeat wash as in step 2. Count viable cells and … black 2 release date pokemonWebAdd 2ml of 1X RBC lysis solution to whole blood/antibody mixture. Incubate at room temperature for 10 minutes. Centrifuge at 350xg for 5 minutes, discard the supernatant. … black 2 team builderWebJan 21, 2024 · Cell Stem Cell. 2024;27:876-889.e12 pubmed publisher Krivanek J, Soldatov R, Kastriti M, Chontorotzea T, Herdina A, Petersen J, et al . Dental cell type atlas reveals … black 2 shiny codeWebWash cells twice with cold BioLegend cell staining buffer (cat # 420241) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10 6 … black2thefuture.org